Peltier cooling (theknoloji ea ho pholisa ea thermoelectric e ipapisitseng le phello ea Peltier) e fetohile e 'ngoe ea mahlale a mantlha a sistimi ea taolo ea mocheso bakeng sa lisebelisoa tsa PCR (polymerase chain reaction) ka lebaka la karabelo ea eona e potlakileng, taolo e nepahetseng ea mocheso, le boholo ba komporo, e susumetsang ts'ebetso, ho nepahala, le maemo a ts'ebeliso ea PCR. Lintlha tse latelang ke tlhahlobo e felletseng ea lits'ebetso tse ikhethileng le melemo ea pholiso ea thermoelectric(peltier cooling) ho qala ka litlhoko tsa mantlha tsa PCR:
Litlhoko tsa I. Core bakeng sa Taolo ea Mocheso ho PCR Technology
Ts'ebetso ea mantlha ea PCR ke potoloho e pheta-phetoang ea denaturation (90-95 ℃), annealing (50-60 ℃), le katoloso (72 ℃), e nang le litlhoko tse thata haholo bakeng sa sistimi ea taolo ea mocheso.
Ho phahama le ho theoha ha mocheso ka potlako: Ho khutsufatsa nako ea potoloho e le 'ngoe (mohlala, ho nka metsotsoana e seng mekae feela ho theoha ho tloha 95℃ ho ea ho 55℃), le ho ntlafatsa katleho ea karabelo;
Taolo e nepahetseng ea mocheso o phahameng: Ho kheloha ha ± 0.5 ℃ mochesong oa annealing ho ka lebisa ho holisa ka mokhoa o sa tobang, 'me e lokela ho laoloa ka har'a ± 0.1℃.
Ho lumellana ha mocheso: Ha lisampole tse ngata li sebetsa ka nako e le 'ngoe, phapang ea mocheso pakeng tsa sampole ea Wells e lokela ho ba ≤0.5℃ ho qoba ho kheloha ha sephetho.
Ho ikamahanya le maemo a miniaturization: PCR e nkehang (joalo ka maemo a POCT a sebakeng sa marang-rang) e lokela ho ba e kopaneng ka boholo 'me e se na likarolo tsa mochini.
II. Lisebelisoa tsa mantlha tsa pholiso ea thermoelectric ho PCR
Thermoelectric Cooler TEC, Thermoelectric cooling module, peltier module e finyella "phetoho ea bobeli ea ho futhumatsa le ho pholisa" ka ho toba hona joale, e lumellanang hantle le litlhoko tsa taolo ea mocheso oa PCR. Tšebeliso ea eona e khethehileng e bonahala likarolong tse latelang:
1. Ho phahama le ho theoha ha mocheso ka potlako: Ho khutsufatsa nako ea ho itšoara
Molao-motheo: Ka ho fetola tataiso ea mochine oa hona joale, oa TEC, mochine oa thermoelectric, sesebelisoa sa peltier se ka fetoha ka potlako pakeng tsa "ho futhumatsa" (ha nako e ntse e tsoela pele, qetello ea mocheso ea TEC module, peltier module e fetoha qetello e lokollang mocheso) le "ho pholile" (ha nako ea hona joale e fetoha, nako e fokolang ea mocheso e fetoha nako e fokolang ea mocheso, hangata e fetoha nako e fokolang ea mocheso). 1 motsotsoana.
Melemo: Mekhoa ea khale ea ho etsa sehatsetsi (joalo ka li-fan le li-compressor) e itšetlehile ka ho tsamaisa mocheso kapa motsamao oa mochine, 'me likhahla tsa ho futhumatsa le ho pholisa hangata li ka tlase ho 2℃/s. Ha TEC e kopantsoe le li-block tsa tšepe tse phahameng tsa mocheso (tse kang koporo le aluminium alloy), e ka finyella tekanyo ea ho futhumatsa le ho pholisa ea 5-10 ℃ / s, ho fokotsa nako e le 'ngoe ea potoloho ea PCR ho tloha metsotso ea 30 ho ea ho metsotso e ka tlase ho 10 (joalo ka lisebelisoa tse potlakileng tsa PCR).
2. Ho laola mocheso o nepahetseng ka ho fetisisa: Ho netefatsa hore ho na le lintlha tse khethehileng tsa amplification
Molao-motheo: Matla a tsoang (ho futhumatsa / ho pholile) a mojule oa TEC, mojule oa pholiso ea thermoelectric, mojule oa thermoelectric o tsamaisana hantle le matla a hajoale. E kopantsoe le li-sensor tse phahameng tsa mocheso (tse kang ho hanyetsa platinum, thermocouple) le tsamaiso ea ho laola maikutlo a PID, hona joale e ka fetoloa ka nako ea sebele ho finyella taolo e nepahetseng ea mocheso.
Melemo: Ho nepahala ha taolo ea mocheso ho ka fihla ± 0.1 ℃, e leng holimo haholo ho feta bateng ea setso sa mokelikeli kapa sehatsetsi sa compressor (± 0.5 ℃). Mohlala, haeba thempereichara nakong ea sethala sa annealing e le 58 ℃, mojule oa TEC, mochini oa thermoelectric, peltier cooler, peltier element e ka boloka mocheso ona ka mokhoa o tsitsitseng, ho qoba ho tlama li-primers ka lebaka la ho feto-fetoha ha mocheso le ho ntlafatsa haholo boleng ba amplification.
3. Moralo oa Miniaturized: Ho khothaletsa nts'etsopele ea PCR e nkehang
Molao-motheo: Bophahamo ba module ea TEC, karolo ea peltier, sesebelisoa sa peltier ke lisenthimithara tse seng kae feela (mohlala, 10 × 10mm TEC module, thermoelectric cooling module, peltier module e ka finyella litlhoko tsa sampuli e le 'ngoe), ha e na likarolo tse tsamaeang (tse kang piston ea compressor kapa li-fan blades), 'me ha e hloke lihatsetsi.
Melemo: Ha lisebelisoa tsa PCR tsa setso li itšetleha ka li-compressor bakeng sa ho pholisoa, molumo oa tsona hangata o feta 50L. Leha ho le joalo, lisebelisoa tsa PCR tse nkehang tse sebelisang mochine o futhumatsang oa thermoelectric, module ea thermoelectric, peltier module, module ea TEC e ka fokotsoa hore e be ka tlase ho 5L (joaloka lisebelisoa tse tšoaroang ka letsoho), e leng se etsang hore li tšoanelehe bakeng sa tlhahlobo ea tšimo (e kang ho hlahloba setšeng nakong ea mafu a seoa), tlhahlobo ea kliniki e haufi le bethe, le maemo a mang.
4. Mocheso o ts'oanang: Etsa bonnete ba ho lumellana har'a mehlala e fapaneng
Molao-motheo: Ka ho hlophisa lihlopha tse ngata tsa TEC (tse kang 96 micro TECs tse lumellanang le poleiti ea seliba sa 96), kapa hammoho le li-blocks tsa tšepe tse arolelanang mocheso (lisebelisoa tse phahameng tsa mocheso oa mocheso), ho kheloha ha mocheso ho bakoang ke ho se tšoane ha batho ka bomong ho TEC ho ka fokotsoa.
Melemo: Phapang ea mocheso lipakeng tsa sampole Wells e ka laoloa ka har'a ± 0.3 ℃, ho qoba liphapang tse sebetsang hantle tsa amplification tse bakoang ke mocheso o sa tsitsang lipakeng tsa Wells tse bohareng le Wells bohareng, le ho netefatsa ho bapisa ha sephetho sa sampole (joalo ka ho ts'oana ha litekanyetso tsa CT ho PCR ea nako ea nnete ea fluorescence quantitative).
5. Ho tšepahala le ho boloka nako: Fokotsa litšenyehelo tsa nako e telele
Molao-motheo: TEC ha e na likarolo tsa ho roala, e na le nako ea bophelo ea lihora tse fetang 100,000, 'me ha e hloke hore lihatsetsi li nkeloe sebaka khafetsa (tse kang Freon in compressors).
Melemo: Kakaretso ea nako ea bophelo ba sesebelisoa sa PCR se pholileng ke compressor ea setso e ka ba lilemo tse 5 ho isa ho tse 8, ha tsamaiso ea TEC e ka e atolosa ho feta lilemo tse 10. Ho feta moo, tlhokomelo e hloka feela ho hloekisa mocheso oa mocheso, ho fokotsa haholo litšenyehelo tsa ts'ebetso le tlhokomelo ea thepa.
III. Mathata le Ntlafatso ea Likopo
Pholiso ea semiconductor ha e ea nepahala ho PCR mme e hloka ntlafatso e lebisitsoeng:
Mocheso o thibelang mocheso: Ha TEC e pholile, mocheso o mongata o bokella qetellong ea mocheso (mohlala, ha mocheso o theoha ho tloha 95 ℃ ho ea ho 55 ℃, phapang ea mocheso e fihla ho 40 ℃, 'me matla a ho lokolla mocheso a eketseha haholo). Hoa hlokahala ho e kopanya le mokhoa o sebetsang hantle oa ho senya mocheso (joalo ka li-sinks tsa mocheso oa koporo + li-fans tsa turbine, kapa li-modules tsa ho futhumatsa metsi), ho seng joalo ho tla lebisa ho fokotseha ha katleho ea ho pholile (esita le tšenyo e feteletseng).
Taolo ea tšebeliso ea matla: Tlas'a liphapang tse kholo tsa mocheso, tšebeliso ea matla ea TEC e batla e phahame (mohlala, matla a TEC a sesebelisoa sa 96-well PCR a ka fihla ho 100-200W), 'me hoa hlokahala ho fokotsa tšebeliso e sa sebetseng ea matla ka li-algorithms tse bohlale (tse kang ho laola mocheso oa mocheso).
Iv. Maemo a Sebetsang a Kopo
Hajoale, lisebelisoa tsa mantlha tsa PCR (haholo-holo lisebelisoa tsa PCR tsa nako ea 'nete tsa fluorescence) ka kakaretso li amohetse theknoloji ea pholiso ea semiconductor, mohlala:
Thepa ea boemo ba laboratori: Sesebelisoa sa PCR se nang le 96-well fluorescence quantitative quantitative ea mofuta o itseng, se nang le taolo ea mocheso oa TEC, se futhumatsang le ho pholisa ho fihla ho 6 ℃/s, taolo ea mocheso e nepahetseng ea ± 0.05 ℃, 'me e tšehetsa 384-ho lemoha ha mocheso o phahameng.
Sesebelisoa se nkehang habobebe: Sesebelisoa se itseng sa PCR se tšoaroang ka letsoho (sa boima bo ka tlase ho 1kg), se ipapisitse le moralo oa TEC, se ka phethela ho fumanoa ha coronavirus e ncha nakong ea metsotso e 30 mme se loketse maemo a setšeng joalo ka boemafofane le sechaba.
Kakaretso
Pholiso ea Thermoelectric, e nang le melemo e meraro ea mantlha ea karabelo e potlakileng, ho nepahala ho phahameng le miniaturization, e rarollotse lintlha tsa bohloko tsa theknoloji ea PCR mabapi le ts'ebetso, boits'oaro le maemo a maemo, ho fetoha theknoloji e tloaelehileng ea lisebelisoa tsa sejoale-joale tsa PCR (haholo-holo lisebelisoa tse potlakileng le tse nkehang habonolo), le ho nts'etsapele PCR ho tloha laboratoring ho ea libakeng tse pharaletseng tsa ts'ebeliso joalo ka bethe ea kliniki.
TES1-15809T200 bakeng sa mochini oa PCR
Mocheso oa lehlakoreng le chesang: 30 C,
Boima: 9.2A,
Boholo: 18.6V
Khōlo: 99.5 W
Delta T boholo: 67 C
ACR: 1.7 ±15% Ω (1.53 ho 1.87 Ohm)
Boholo: 77×16.8×2.8mm
Nako ea poso: Aug-13-2025
