Tšebeliso ea theknoloji ea ho pholisa ea thermoelectric lisebelisoa tsa PCR

Tšebeliso ea theknoloji ea ho pholisa ea thermoelectric lisebelisoa tsa PCR

Tšebeliso ea theknoloji ea ho pholisa ea thermoelectric lisebelisoa tsa PCR e itšetlehile haholo-holo taolong ea mocheso. Molemo oa eona oa mantlha ke bokhoni ba ho laola mocheso ka potlako le ka nepo, bo netefatsang sekhahla sa katleho sa liteko tsa ho eketsa DNA.

Maemo a bohlokoa a ts'ebeliso

1. Taolo e nepahetseng ea mocheso

Sesebelisoa sa PCR se hloka ho potoloha mekhahlelo e meraro: ho fetoha ha mocheso o phahameng (90-95℃), ho annealing mochesong o tlase (55-65℃), le katoloso e ntle ea mocheso (70-75℃). Mekhoa ea setso ea sehatsetsing e thata ho fihlela tlhoko ea ho nepahala ea ±0.1℃. Theknoloji ea ho pholisa ea thermoelectric cooling, peltier cooling e fihlella taolo ea mocheso oa boemo ba millisecond ka phello ea Peltier, e qoba ho hloleha ha keketseho ho bakoang ke phapang ea mocheso oa 2℃.

2. Ho pholisa le ho futhumatsa ka potlako

Dimojule tsa ho phodisa tsa thermoelectric, dimojule tsa thermoelectric, disebediswa tsa peltier, dimojule tsa peltier di ka fihlella sekgahla sa ho phodisa sa di-degrees Celsius tse 3 ho isa ho tse 5 ka motsotsoana, e leng se kgutsufatsang potoloho ya teko haholo ha e bapiswa le di-degrees Celsius tse 2 ka motsotsoana tsa di-compressor tsa setso. Mohlala, sesebediswa sa PCR sa didiba tse 96 se sebedisa theknoloji ya taolo ya mocheso wa zonal ho netefatsa dithempereichara tse tsitsitseng maemong ohle a didiba le ho qoba phapang ya mocheso wa 2℃ e bakwang ke ditlamorao tsa bohale.

3. Ntlafatsa ts'epo ea lisebelisoa

Dimojule tsa thermoelectric cooling, dimojule tsa peltier, dimojule tsa peltier, dimojule tsa TEC tsa Beijing Huimao Cooling Equipment Co., Ltd. di fetohile dikarolo tsa mantlha tsa taolo ya mocheso wa disebediswa tsa PCR ka lebaka la ho tshepahala ha tsona ho hoholo. Boholo ba yona bo bonyenyane le dikarolo tse se nang lerata di etsa hore e lokele ditlhoko tse nepahetseng tsa disebediswa tsa bongaka.

Maemong a tloaelehileng a kopo

Sesebediswa sa PCR sa fluorescence sa 96-well: Se kopantswe le module ya thermoelectric cooling, module ya TEC, sesebediswa sa peltier, dimodule tsa peltier. E nolofalletsa taolo e nepahetseng ya mocheso wa disampole tse nang le phallo e phahameng mme e sebediswa haholo masimong a kang tlhahlobo ya tlhahiso ya lefutso le ho lemoha pathogen.

Lihatsetsi tsa bongaka tse nkehang habobebe: ho pholisa ka thermoelectric, ho pholisa ka peltier lihatsetsi tsa bongaka tse nkehang habobebe tse sebelisetsoang ho boloka lihlahisoa tse kang liente le meriana e hlokang tikoloho e mocheso o tlase, ho netefatsa botsitso ba mocheso nakong ea lipalangoang.

Lisebelisoa tsa kalafo ea laser:

Dimojule tsa ho phodisa tsa thermoelectric, dielemente tsa peltier, dimojule tsa thermoelectric di phodisa seemedi sa laser ho fokotsa kotsi ya ho tjha ha letlalo le ho ntlafatsa polokeho ya kalafo.

Tlhaloso ea TEC1-39109T200

Mocheso o chesang oa lehlakore ke 30 C,

Setšoantšo: 9A

Matla a phahameng: 46V

Qmax: 246.3W

ACR: 4±0.1Ω（Ta= 23 C）

Tekanyo e phahameng ea Delta T: 67 -69C

Boholo: 55x55x3.5-3.6mm

Tlhaloso ea TES1-15809T200

Mocheso o chesang oa lehlakore: 30 C,

Tekanyo e phahameng: 9.2A,

Matla a phahameng: 18.6V

Qmax: 99.5 W

Tekanyo e phahameng ea Delta T: 67 C

ACR：1.7 ± 15% Ω （1.53 ho isa ho 1.87 Ohm）

Boholo: 77×16.8×2.8mm

Terata: Terata ea silicone ea 18 AWG kapa e lekanang le eona e koahetsoeng ka Sn holim'a metsi, Khanyetso ea mocheso o phahameng 200℃